For microdissection the arcturusxt laser capture microdissection system thermo scientific and arcturus capsure macro lcm caps life technologies were used. Laser capture microdissection lcm is a method to procure subpopulations of tissue cells under direct microscopic visualization. Laser capture microdissection under a microscope using a software interface a tissue section typically 5 50 micrometres thick is viewed and individual cells or clusters of cells are identified either manually or in semi automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation.
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